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During an investigation of a foodborne outbreak linked to a church supper, 80 people ate the suspected chicken dish, and 32 subsequently developed gastroenteritis. Among 50 people who did not eat the chicken dish, 5 developed similar symptoms. What is the attack rate among those who ate the chicken dish? 

A Gram-positive rod is isolated from the blood culture of a neonate with sepsis. The organism exhibits weak beta-hemolysis on sheep blood agar, is catalase-positive, hydrolyzes esculin in the presence of bile, and demonstrates characteristic "tumbling" motility in a wet mount prepared from a broth culture incubated at 25° C. Which organism is most likely? 

Metagenomic analysis of a bronchoalveolar lavage (BAL) sample from a patient with severe pneumonia reveals a complex microbial community with a high abundance of both bacterial and fungal sequences. This approach is particularly useful in identifying:

A clinical microbiology laboratory is investigating a cluster of Clostridium difficile infections in a hospital. Which of the following methods is MOST appropriate for determining if the isolates from different patients are related?

A clinical microbiology laboratory is using flow cytometry to analyze lymphocyte subsets in a patient with suspected immunodeficiency. Which of the following cell surface markers is typically used to identify helper T cells?